Technische Universität München, Germany 2008 - 2011
(3 )
出版物
Extension of Helix 12 in Munc18-1 Induces Vesicle Priming
A. S. Munch, G. H. Kedar...Timon André...T. H. Söllner, M. Verhage, and J. B. Sørensen
Our findings support the notion that a conformational transition within the Munc18-1 domain 3a helix 12 leads to opening of a closed Munc18-1:syntaxin complex, followed by productive SNARE complex assembly and vesicle priming.
MicroScale Thermophoresis: Interaction analysis and beyond
M. Jerabek-Willemsen, Timon André, R. Wannera, H M Roth, S. Duhr, P. Baaske, D.Breitsprecher
MicroScale Thermophoresis (MST) is a powerful technique to quantify biomolecular interactions. It is based on thermophoresis, the directed movement of molecules in a temperature gradient, which strongly depends on a variety of molecular properties such as size, charge, hydration shell or conformation.