Molecular biology questions involving research & critical thinking
$10-30 USD
着払い
Seeking anyone who has completed a PhD in preferably molecular biology or any biological science for answering, using research & critical thinking, any 2 of these questions:
1. Expression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use. In an attempt to get a new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. Will this strategy result in good expression of gene X? Why or why not?
3. You obtain the sequence of a gene containing 10 exons, 9 introns, and a 3_ UTR containing a polyadenylation consensus sequence. The fifth intron also contains a polyadenylation site. To test whether both polyadenylation sites are used, you isolate mRNA and find a longer transcript from muscle tissue and a shorter mRNA transcript from all other tissues. Speculate about the mechanism involved in the production of these two transcripts.
4. You are investigating the transcriptional regulation of genes from a recently discovered eukaryotic organism. You find that this organism has three RNA polymerase–like enzymes and that genes encoding proteins are transcribed using
an RNA polymerase II–like enzyme. When examining the full-length mRNAs produced, you find that there is no 5 structure (i.e., cap). Are you surprised? Why/why not?
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